Investigator Lead: Prof. Julian Hibberd
Participating Laboratories: Cambridge, Berkeley, Exeter, Warwick, Glasgow, MSU
Efficiency gains in substrate channelling arise from physical proximity that enables (near-) direct transfer of intermediates from one enzymatic step to the next, thereby minimizing runoff of intermediates between steps. Synthetic scaffolding allows protein stoichiometries to be tailored on the scaffold so that flux-control coefficients can be matched across the metabolic pathway.
The second component of our strategy is the design and implementation of a scaffold to channel and retain inorganic carbon once transported into the chloroplast. We have for the first time shown that it is possible to express and assemble artificial protein scaffolds in both cyanobacteria and in the stroma of chloroplasts.
Various strategies are underway to explore assemblies and thier coordination, including bimolecular fluorescence complementation in vivo and biochemical pulldown assays in vitro.